EKSTRAKSI KOMPONEN BIOAKTIF SERBUK KAYU SECANG (Caesalpinia sappan, L) DENGAN METODE ULTRASONIKASI

Neswati Neswati, Sahadi Didi Ismanto

Sari

Komponen bioaktif dari kayu secang (Caesalpinia sappan, L) dapat dimanfaatkan sebagai antibakteri dan antioksidan. Komponen bioaktif tersebut dapat diekstraksi dengan menggunakan metode ultrasonikasi yang diberi perlakuan jenis pelarut (air dan etanol), suhu ekstraksi (20°C, 30°C dan 40°C) dan lama ekstraksi (20 menit, 30 menit dan 40 menit). Adapun parameter yang diuji yaitu antibakteri Staphylococcus aureus dengan metode zona bening, aktivitas antioksidan IC 50, total fenol, dan total flavanoid. Hasil penelitian menunjukkan bahwa kondisi optimum ektraksi kayu secang dengan metode ultrasonikasi adalah menggunakan pelarut etanol 65% pada suhu 30°C selama 40 menit dengan parameter yaitu kadar flavonoid sebesar 0,0667±0,0053 mg QE/g sampel, kadar total fenol sebesar 38,7500±3,6534 mg GAE/g sampel, antibakteri Staphylococcus aureus sebesar 23,25±1,2021mm, dan aktivitas antioksidan IC50 sebesar 1,2978±0,0013 mg/mL.

 Kata Kunci

caesalpinia sappan, L.; ekstrasi; komponen bioaktif; ultrasonikasi

 Teks Lengkap:

PDF (English)

Referensi

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DOI: https://doi.org/10.25077/jtpa.22.2.187-194.2018

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